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KMID : 0848120120370030130
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International Journal of Oral Biology
2012 Volume.37 No. 3 p.130 ~ p.136
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Fusobacterium nucleatum GroEL signaling via Toll-like receptor 4 in human microvascular endothelial cells
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Lee Hae-Ri
Choi Bong-Kyu
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Abstract
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The GroEL heat-shock protein from Fusobacterium nucleatum,a periodontopathogen, activates risk factors foratherosclerosis in human microvascular endothelial cells(HMEC-1) and ApoE-/- mice. In this study, we analyzedthe signaling pathways by which F. nucleatum GroELinduces the proinflammatory factors in HMEC-1 cellsknown to be risk factors associated with the development ofatherosclerosis and identified the cellular receptor used byGroEL. The MAPK and NF-κB signaling pathways were foundto be activated by GroEL to induce the expression of interleukin-8 (IL-8), monocyte chemoattractant protein 1 (MCP-1), intercellular adhesion molecule 1 (ICAM-1), vascularcell adhesion molecule 1 (VCAM-1), E-selectin, and tissuefactor (TF). These effects were inhibited by a TLR4knockdown. Our results thus indicate that TLR4 is a keyreceptor that mediates the interaction of F. nucleatumGroEL with HMEC-1 cells and subsequently induces an inflammatoryresponse via the MAPK and NF-κB pathways.
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KEYWORD
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Fusobacterium nucleatum, GroEL, Periodontitis, Signaling
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